Cell proliferation assay for hMSCs in various hepatogenic culture conditions.

<p>(A) <i>In vitro</i> cytotoxicity assay showing MTT activity across 9 days of induction in different culture conditions. Error bars represents mean ± S.D. The differences in cell proliferation between the groups were considered statistically significant at <i>p</i><0.05 and <i>p</i>-values are indicated on the graph. (*) indicates significant increase in cell proliferation at day 9 compared to day 3 and day 6 in 5% patient sera group. (#) indicates no significant difference in hMSC proliferation at different time points across 9 days of culture. (B) Cell proliferation assay by direct cell counting reveals higher proliferation rate in 10% FBS, which was comparable to that of 10% normal sera (NS) induction group. 10% patient sera (PS) induction group did not show any increase in cell proliferation. However, 5% PS group showed increase in proliferation, which was significantly more compared to 10% PS group (*<i>p</i><0.05). (C) Cell death assay by annexinV-FITC/PI flow cytometric quantification revealed that 10% patient sera caused mostly necrotic cell death with increase in cell death across 9 days, whereas in 5% PS group cell death was comparably minimal. However, there was negligible cell death in control groups.