The proliferation of SH-SY5Y cells was accelerated in the HMF.

<p><b>A:</b> Cell proliferation assay by CCK-8 kit corresponding to the treatments in (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054775#pone-0054775-g002" target="_blank">Figure 2</a>) (n = 6). <b>B:</b> Cells were seeded at 2.0×10⁴/cm² in 6-well plates and cell proliferation was measured by crystal violet staining after 48 h incubation in the GMF and HMF (n = 6). <b>C:</b> Cells were seeded at 2.0×10⁴/cm² in 60 mm petri dishes and incubated for 48 h in the GMF and HMF. The numbers of SH-SY5Y cells were measured at day 1, day 2, and day 3 by hematocytometery (n = 3). <b>D:</b> Cells were seeded at 1.5×10⁴ cells/cm² in 96-well plates. Cell proliferation was measured after 48 h incubation in the reference field (GMF’), in the GMF control shelf (GMF), and in the HMF (n = 6). Error bar = s.d.; n = 3; *p<0.05; **p<0.01.