Assessment of hepatic trans-differentiation efficiency of hMSCs.

<p>Representative flow cytometry data showing average number of albumin positive cells (A) and endoglin (CD105) positive cells (B) after hepatic trans-differentiation of hMSCs in hepatogenic cocktail induction group (i), patient sera induction group (ii) and normal sera induction group (iii). Untreated hMSCs grown in FBS (iv) and HepG2 cells (v) were used as negative and positive control respectively. Histograms showing isotype control (blue area, quantified by M1) and the positive expression of either albumin or endoglin (green area, quantified by M2). Results are representative of three different experiments. Comparison of the total number of albumin positive cells (C) and endoglin positive cells (D) in different hepatogenic culture conditions as well as controls represents the trans-differentiation efficiency. Differences in cell number between the groups were considered statistically significant at <i>p</i>< 0.05 and <i>p</i>-values are indicated on the graph.