Geldanamycin analogues limit high-fat diet-induced adipose tissue expansion.

<p>(A to F) Two groups of mice HFD (n = 7) and HFD+17-DMAG (n = 8) were fed a HFD for 33 days and daily injected intraperitoneally with PBS or with 10 mg/kg of BW 17-DMAG. One group as control ND (n = 4) were fed with standard chow. (A) Percentage of body weight (BW) gain was measured. (B) Body composition of the mice fed with HFD or HFD+17-DMAG was evaluated by EchoMRI. (C) Histological analysis of the epididymal adipose tissue fixed and stained with hematoxylin and eosin. (D) Adipocytes cell surface was calculated using the ImageJ software, ***p<0.001. (E) White adipose tissue lysates were submitted to immunoblotting using antibodies against PPARγ, CEBPα and actin as a loading control. (F) The protein expression levels were quantified and values are given as mean ± SEM (n = 7 HFD, n = 8 HFD+17-DMAG), **p<0.01.