Transfection of lymphatics with ca-ROCK protein increases tone.

<p>Isolated, fura-2 loaded rat mesenteric lymphatics were treated with ca-ROCK protein (final concentration 2 μg/ml) combined with TransIT-LT1 (8 μl/ml final), or with the same amount of TransIT-LT1 alone (Vehicle). <i>A.</i> Mean EDD/MaxD. <i>B.</i> Mean ESD/MaxD. <i>C.</i> Mean AMP/MaxD. <i>D.</i> Mean tone. <i>E.</i> Mean CF. <i>F.</i> Mean diastolic 340/380 ratio. <i>G</i>. Mean change in the 340/380 ratio during Ca²⁺ transients. *P<0.05 versus baseline (BL), same treatment group. N = 5 vessels for the ca-ROCK group, and N = 6 vessels for the vehicle group.