Endogenous M-LECP promote inflammatory and tumor lymphangiogenesis <i>in vivo</i>.

<p>(A, B) C57BL/6 female mice were subjected to a lethal dose of radiation and injected with BM cells from GFP transgenic mice. Once BM reconstitution was complete (4 weeks later), mice received daily injections of LPS (50 μg) of for two weeks to induce chronic peritonitis. At this time, diaphragms were removed and stained with antibodies to GFP and <b>(</b>A) Lyve-1 or (B) Meca-32. (C) EMT6-Luc tumor bearing mice were injected with 5x10⁶ of GFP⁺/CD11b⁺/Pdpn^- or GFP⁺/CD11b⁺/Pdpn⁺ BM cells (6–7 mice per group). EMT6-Luc tumors were harvested 14 days later and stained with antibodies to Lyve-1 and GFP. Images were acquired at 400X magnification. (D) Recruitment of CD11b⁺/Pdpn^- and CD11b⁺/Pdpn⁺ monocytes to EMT6-Luc tumors was quantified by determining the number of GFP⁺ pixels per field from four fields per section. (E) Peritumoral LVD of EMT6-Luc tumors was calculated by counting all peritumoral Lyve-1⁺ vessels on a section and normalizing per section area. Square and dot symbols represent Pdpn-positive and Pdpn-negative CD11b⁺ cells, respectively. Each symbol represents a value obtained from an individual mouse and reports the percentage of field covered by GFP cells or the density of Lyve-1⁺ vessels, respectively. The black bar indicates the mean for each group. Statistical significance was calculated by Student’s t-test with P-value indicated above black bracket.