TdTomato/Luc expression by LL/2 cells increases tumor immunogenicity and inhibits tumor-induced G-CSF and MDSC levels.

(A-C) Tumors from Fig 2B were harvested and analyzed for tumor infiltrating cell counts of (A) CD4+ T cells (CD45+, CD3+, CD4+), (B) CD8+ T cells (CD45+, CD3+, CD8+), and (C) B cells (CD45+, CD19+) are shown. (D-F) MDSC levels in the spleen, tumor and blood samples of LL/2 and LL/2-tdTomato/Luc tumor bearing mice. (G) The levels of common MDSC-inducing factors in the plasma of mice including G-CSF, GM-CSF, TNF-α, IFN-γ, IL-1β, and IL-6. (H) The concentration of G-CSF in the plasma of mice. Mean ± SEM is plotted. Significance of differences was determined by using unpaired student t-test. Values of p<0.05 were considered significant (*p < 0.05, **p < 0.01, ***p ≤ 0.001).