NLGP downregulates suppressor cells within TME.

<p>Single cell suspension was prepared from day 20 tumors of PBS and NLGP treated mice. <b>A.</b> Proportion of activated T cells <b>B.1.</b> CD4⁺CD25⁺ cells, <b>B.2.</b> Tregs, <b>C.</b> and MDSCs within tumors from these two groups of mice was determined flow cytometrically after labeling cells with fluorescence tagged CD8, CD69, CD4, CD25 and CD11b, GR1 antibodies. *<i>p</i><0.001, **<i>p</i><0.05. <b>D.1.</b> Total protein was isolated from tumors at different days and level of Foxp3 was analyzed by immunoblot analysis. <b>D.2.</b> Densitometric analysis was performed in each case. <b>E.1.</b> Total RNA was also purified to assess the expression status of CTLA4 and IDO on different time points of tumor growth. <b>E.2.</b> Densitometric analysis was performed in each case.