Increased peritoneal tumor seeding in LPP1 KO mice.

<p>ID8ip2Luc ovarian cancer cells were injected IP into C57BL/6 (WT) or LPP1 KO mice. <b>(A)</b> Mice (WT n = 10; LPP1 KO n = 12) were imaged weekly following tumor initiation to monitor tumor growth. Data represent mean total flux (photons/second) ± std err and were analyzed by 2-way ANOVA followed by Tukey’s multiple comparisons test. <b>(B)</b> The total number of microscopic tumor nodules was counted in an individual, randomly selected, H&E stained section of omentum per mouse obtained 2 weeks after tumor initiation (WT n = 7; LPP1 KO n = 8). Data represent mean ± std err; p = 0.0069 determined by Student’s t-test. <b>(C)</b> The percentage of wild type or LPP1 KO (from <b>(B)</b>) mice with invasive (solid bars) or non-invasive (includes no tumor; stippled bars) tumors 2 weeks after tumor initiation. Number of mice per outcome is indicated. Significance determined by Fisher’s Exact Test. <b>(D)</b> The percentage wild type or LPP1 KO (from <b>(B)</b>) mice with (positive, solid bars) or without (negative, stippled bars) mesothelial VCAM-1 staining by IHC 2 weeks after tumor initiation. Number of mice per outcome is indicated. Significance determined by Fisher’s Exact Test.