Tumorigenicity in young A53T α-Syn +/+ and control F1 B6/C3H mice.

<p>(a). Mice (3–4 months old) were subcutaneously injected with 0.25×10⁶ B16 melanoma cells. Tumor volumes were determined every 1–2 days post injection. The experiment was stopped after 15 days, when the tumors in the A53T α-Syn +/+ group reached 1 to 1.2 cm in diameter. The graph represents the means ± standard errors (SE) of 5–8 mice in each group. A representative growth curve out of three independent experiments. (b). Mice were injected with 1×10⁵ E0771 mammary gland adenocarcinoma cells and tumor volume was measured as in (a). (c). Mice were injected with 5×10⁴ D122 Lewis lung carcinoma and tumor volume was measured as in (a). (d). Mean weights of tumors formed in each genotype group at 15 days post- injection ± SE for B16, *,p = 0.015, t-test; (e). E0771, *, p = 0.012, t-test; (f). D122; (g). Mean weights of tumors formed in each genotype group at 15 days post- injection ± SE, n = 6–7, *, p<0.05 Mann-Whitney test.