Accumulation and infiltration of iPS-ML in pre-established tumor tissues in mouse peritoneal cavity.

<p><b>A.</b> GFP-expressing NUGC-4 cells (5 ×10⁶ cells/mouse) were injected into the peritoneal cavity of SCID mice. After 15 days, iPS-ML labeled with fluorescent PKH26 were injected i.p. into the mice (3×10⁶ cells/mouse). The mice were sacrificed the following day and subjected to fluorescence imaging analysis to determine the location of the NUGC-4-GFP tumors (excitation/emission: 475/520 nm) and PKH26-iPS-ML (excitation/emission: 550/600 nm). <b>B.</b> Tumor tissues in the greater omentum of the mice were isolated, and 20-µm thick frozen sections were made. The sections were analyzed on a fluorescence microscope, and a merged image with green fluorescence indicating NUGC-4/GFP cells and red fluorescence indicating PKH26-stained iPS-ML is shown. <b>C, D</b> Tissue sections were made by a similar procedure as for <b>B</b>, except that tPA was not used. A higher magnification view of the region surrounded by a dotted square in <b>C</b> is shown in <b>D</b>.