mTORC2 loss exacerbates the pro-inflammatory profile of BMDM in response to several TLR agonists.

<p>(A to D) Impact of several TLR agonists on gene expression in macrophages isolated from LysM-<i>Rictor</i>^WT or LysM-<i>Rictor</i>^KO mice. BMDM were isolated from LysM-<i>Rictor</i>^WT or LysM-<i>Rictor</i>^KO mice and were differentiated for 6 days <i>in vitro</i>. Cells were then plated and incubated 24 hours. Cells were then treated with (A) PAM3 (1ug/ml), (B) MALP2 (0.1ug/ml), (C) R848 (0.1uM), or CpG (0.5uM) for 8 hours. mRNA expression of markers of the classically activated (M1) and alternatively activated (M2) macrophages were measured by qRT-PCR and normalized to 36B4 mRNA levels. Data are expressed as the mean ± SEM for n = 4 per condition. *p<0.05 versus control. This experiment was repeated twice and similar results were observed.