Gene expression alterations in bone marrow-derived macrophages (BMDM) from Bmpr2^delx4+ mice compared to controls.

<p>Shaded areas indicate SEM; all results are normalized to HPRT expression and then to control baseline. (<b>A</b>) Classical activation marker MRC1 is increased in BMDM from Bmpr2^delx4+ mice. (<b>B</b>) Alternative activation marker Itgam is decreased in BMDM from Bmpr2^delx4+ mice. (<b>C</b>) Canonical BMP pathway target Id1 is strongly suppressed by inflammation. (<b>D</b>) Secreted BMP pathway inhibitor Grem1 is induced by inflammation. (<b>E</b>) Intracellular BMP pathway inhibitor Smad6 is suppressed by inflammation. (<b>F</b>) In combination, these suggest that the BMP pathway normally suppresses classical macrophage activation; when macrophages are activated, they produce secreted factors to suppress the BMP pathway and intracellular factors to reduce suppression of the pathway.