The pro-inflammatory profile induced by <i>Rictor</i> loss is not associated with consistent modulations in MAP kinase signalling and IKKα/β activation.

<p>(A) Impact of LPS on cell signalling in BMDM isolated from LysM-<i>Rictor</i>^WT or LysM-<i>Rictor</i>^KO mice. BMDM were isolated from LysM-<i>Rictor</i>^WT or LysM-<i>Rictor</i>^KO mice and were differentiated for 6 days <i>in vitro</i>. Cells were then plated, incubated 24 hours and then treated with LPS (250ng/ml) for the indicated times. Proteins were extracted and lysates were analysed by immunoblotting for indicated proteins. (B) Impact of LPS on cell signalling in <i>Rictor</i>^+/+ and <i>Rictor</i>^−/− MEFs. MEFs were plated 24 hours before the experimentation. The next day, cells were treated with LPS (5ug/ml) for the indicate time. Proteins were extracted and lysates were analysed by immunoblotting for indicated proteins. (C–D) Impact of other TLR agonists on cell signalling in BMDM isolated from LysM-<i>Rictor</i>^WT or LysM-<i>Rictor</i>^KO mice. Cells were isolated as described in A and were treated with either (C) R848 or (D) CpG for the indicated times. Protein were extracted and treated as described in A.