Kinetic profiles of signaling pathway activation in response to TLR2 ligands in BMDMs.

<p>Primary bone marrow derived macrophages (BMDMs), isolated from either wildtype or TLR2-/- mice, treated with LPS (100 ng/mL), LTA (1 μg/mL), R-FSL-1 (100 ng/mL, R), S-FSL-1 (100 ng/mL, S), Pam2CSK4 (100 ng/mL, P2), Pam3CSK4 (100 ng/mL, P3) for 0, 5, 15, 30,45, or 60 minutes were used to perform Western blot analysis of A) phospho-SAPK/JNK activation signal and total-SAPK/JNK protein amounts, B) Phospho-p38 activation signal and total-p38 protein amounts, and C) total IκBα protein amounts. In D) RT-PCR was performed on mRNA isolated from wildtype or TLR2-/- bone marrow derived macrophage treated with each ligand for 4 hours and the TLR2-dependence of cell activation leading to TNFα mRNA transcription is shown. All results are representative of n = 3 independent experiments.