Pathways altered at the transcript level by RNase H overexpression.

<p>(A) Gene Ontology term network of genes with increased (left) or decreased (right) mRNA levels upon RNase H overexpression. Representative terms from <b>Supplementary <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004288#pgen.1004288.s023" target="_blank">Table S10</a></b> are shown. Node size indicates fold enrichment. Node color indicates the number of genes associated with each term (the darkest indicating the greatest number of genes associated). Edge thickness indicates the number of genes shared between terms. (B) 10-fold serial dilutions on BPS iron plates testing low iron concentration sensitivity of wild type versus DNA:RNA hybrid forming mutants reveals a lack of cellular iron requirement in RNase H mutant strains.