2D gel separation and blotting with SLC38A6 antibody of the total protein in mouse brain.

<p>A) Insoluble fraction of total protein indicating presence of SLC38A6 with the specific band between 37 and 50 KD. B) Soluble fraction of total protein indicating absence of SLC38A6 with no bands. No unspecific bands were detected in any of the fractions, validating the specificity of the antibody.