Immunoblotting analysis using sequentially fractionated mouse nervous tissue using various novel αS antibodies.

<p>The brainstem and spinal cord of an αS KO, a nTG, a 2-month old unaffected homozygous M83 mice, and a 13-month old motor impaired homozygous M83 mice were sequentially fractionated as described in “Materials and Methods”. 20 μg from the high salt (HS) and high salt/Triton (HS/T) and 10 μg from the SDS/urea (SDS/U) fractions were loaded on 15% polyacrylamide gels and analyzed with various antibodies as described above each blot. The arrows indicates αS. The mobilities of molecular mass markers are indicated on the left.