Assessing Antibody Specificity.

<p>Mouse brain sections were stained by immunohistochemistry with anti-BDNF (A) or Ntrk2 (D) antibodies as positive controls, or with antibody which had been pre-incubated with human recombinant BDNF (B) or Ntrk2 (E) protein, or with normal goat serum as a negative control (C, F). Decreased or absent staining was observed in pre-incubated sections as compared to positive controls (A vs. B; D vs. E). A 2X serial dilution of human recombinant BDNF (G) and truncated Ntrk2 (H) revealed bands of the appropriate sizes by Western Blot. Green: BDNF, brown: Ntrk2, blue: nucleus. Arrowheads: Purkinje cells, Gr: Granular layer, Mol: Molecular layer.