Increased iNKT17 cell population in the thymus and periphery of young NOD mice.

<p><b>A</b>, Thymic cells from 6-wk-old C57BL/6 or NOD female mice were stained with CD1d- tetramers (tet) and then subjected to intracellular staining using anti-RORγt antibodies. The frequency and absolute number of RORγt⁺ cells among tet⁺ cells are shown. Data are presented as mean ± SD and are from 4 experiments where 3 to 4 mice per group were used in each experiment. <b>B</b>, Axillary LN cells (representative of maxillary, pancreatic LN and spleen) from 6-wk-old C57BL/6 or NOD female mice were subjected to surface or intracellular staining. The frequency and absolute number of IL-17⁺, RORγt⁺, and CD103⁺CCR6⁺ cells among tet⁺ cells are shown. Data are presented as mean ± SD and are from 4 experiments where 3 to 4 mice per group were used in each experiment. *p<0.05, using non-parametric Mann-Whitney U test to determine significance.