iNKT17 cells are increased in NOD mice compared with C57BL/6 mice.

<p>Axillary (Ax), maxillary (Max), and pancreatic (p) lymph nodes (LNs), and spleen cells from 12-wk-old C57BL/6 or NOD female mice were stimulated with PMA/Ionomycin in the presence of BFA for 4 hours. Cells were then stained with CD1d-tetramers (tet) and antibodies directed against B220 and IL-17A (IL-17). Representative dot and histogram plots are shown and numbers represent percentages. The frequency (upper histograms) and the absolute number (lower histograms) of IL-17⁺ cells among tet⁺ cells are shown. Data are presented as mean ± SD and are from 5 experiments where 3 to 4 mice per group were used in each experiment. *p<0.05, using non-parametric Mann-Whitney U test to determine significance.