Lymphoid and blood abnormalities in <i>spc</i> mice.

<p>(A) Representative thymi dissected from <i>spc/spc</i> and WT (+/+) littermate mice at 4 weeks of age. Scalebar, 5mm. (B) Representative H/E stained thymic sections from <i>spc/spc</i> and +/+ littermate mice (n = 4). Scalebar, 400 µm. (C) Total thymus cellularity in <i>spc/spc</i> mice and control littermates, (n = 14 per group). (D) Percentage of thymic subpopulations in <i>spc/spc</i> mice and control littermates as determined by flow cytometry after staining of thymocytes with antibodies against CD4 and CD8. Data represent means ± SE from four independent experiments, (n = 13 per group). (E) Percentages of CD4^-CD8^- double negative (DN) subpopulations as determined by flow cytometry after staining of thymocytes with antibodies against CD25 and CD44, in <i>spc/spc</i> and control littermates, (n = 6 per group). (F) Representative spleens dissected from <i>spc/spc</i> and +/+ littermate mice at 4 weeks of age. Scalebar, 5 mm. (G) Total spleen cellularity of <i>spc/spc</i> mice and control littermates, (n = 14 per group). (H) Percentage of splenic subpopulations in <i>spc/spc</i> mice and control littermates as determined by flow cytometry using antibodies against CD4, CD8, B220 (B cells), Gr1 and CD11b (Myeloid). Data represent means ± SE from four independent experiments, (n = 10 per group). (I) Peripheral blood counts of <i>spc/spc</i> mice and control littermates, (n = 7 per group). Controls presented in bar graphs are healthy littermates (+/+ and <i>+/spc</i>).