shRNA targeting of SSX in inhibit the growth of xenografts in SCID mice.

<p>A) DFW melanoma cells stably transfected with SSX-shRNA (SSXi) or with a control shRNA (SSXm) vector, expanded and xenografted in SCID mice. A) Tumor volume determined at the indicated times. B) Tumor volume at the end point of the experiment (21 days). C) Growth curves of xenografts from conditionally SSX-shRNA silenced DFW cells (SSX−) and control shRNA (SSX+) cells. Doxycycline was administered to all mice by subcutaneous insertion of a slow release pellet to mantain steady concentration of doxycycline (10 µM) for 21 days. D) Tumor volume at the end point of the experiment. E) Immunohistochemistry of the tumors visualized in the light microscope using 10x objective, inserts 63x magnification: hematoxillin (HTX), the proliferation marker (ki-67) and β-catenin (β-cat).