MED1 knockdown in combination with fulvestrant treatment dramatically inhibits the growth of orthotopic tumor xenografts.

<p>BT474-tet-shMED1 cells were injected into the fat pad of 5-6 weeks old nude mice. After 3 weeks, mice were randomly divided into four groups (4 mice/group): vehicle control (Veh), or doxycycline (+Dox), fulvestrant (+Ful) and the combination treatment (+Dox/+Ful) and examined for the followings: (<b>A</b>) Tumor volumes were measured twice per week by caliper (n=8). Red arrows indicate the time of fulvestrant treatments. (<b>B</b>) Tumors were harvested and the representative images of tumors in each group were shown. (<b>C</b>) Tumor weights were calculated and shown as a box-plot with median and whiskers from minimum to maximum (n=8). (<b>D</b>) Representative tumor bioluminescence images of each group at indicated time point were shown (*: P< 0.05; **: P< 0.01).