Tumorigenic effect of CXCL17 knockdown in HT-29 cells.

<p>(A) Two candidate sequences for shRNA for knocking down CXCL17 expression (shRNA #1 and #2) and corresponding scrambled sequences were also designed (Scrambled #1 and #2). Stable cell lines were isolated and knockdown levels of CXCL17 mRNA were analyzed using RT-PCR. Upper panel, CXCL17; lower panel, GAPDH as an internal control. (B) CXCL17-knockdown HT-29 cells (1 × 10⁶) were transplanted into the subcutaneous space of SHO mice and tumor size was measured at the indicated time points. There was no significant difference between shRNA #1 and Scrambled #1. ND, not different; P = 0.08 (n = 4), One of two independent experiments with similar results is shown. (C) CD31-positive vessels of tumors in xenografts of CXCL17-knockdown HT-29 cells were counted in five random sections. ND, not different (P = 0.19). One of two independent experiments with similar results is shown.