BDNF enhances anoikis resistance in TrkB-expressing CRC cells.

<p>The upper panel (A) shows the number of anoikis resistant cells by counting viable tumor cells that proliferated non-adherently in low-attachment dishes using a WST-8 reagent (cell viability assay). The lower panel (B) shows the representative flow cytometric data showing the proportion of viable tumor cells (lower left quadrant) and apoptotic tumor cells (lower right quadrant+upper right quadrant). DLD1, SW480, and LoVo cells were treated with medium (control), BDNF (100 ng/ml), K252a (100 nM), or K252a followed by BDNF. The floating tumor cells were subjected to cell viability assay and flow cytometric analysis. Exogenous BDNF enhances anoikis resistance in TrkB-expressing CRC cells (increased viable tumor cells), and K252a inhibits the anoikis resistance of tumor cells. Quantitative analysis was performed for cell viability assay. Each independent experiment was performed six times. Results are presented as mean ±SE. *; P<0.05.