TrkB requires ligand to induce anoikis resistance. A.

<p>MCF7 and T47D cells were stably selected for expression of empty vector (EV) or TrkB. Immunoblot showing TrkB expression, α-tubulin used as loading control. MCF7, <b>B</b>, and T47D, <b>C</b>, cells were plated suspended in increasing concentrations of BDNF or NTF3. Cells were harvested 24 hrs later and apoptosis assayed by cell death ELISA, data normalized to attached condition and shown relative to EV conditions. <i>Columns</i>, mean of three biological replicates, <i>bars</i>, SEM.