TrkB and NTF3 are up-regulated in suspended cells and miR-200c blocks this up-regulation.

<p>Cells were plated in suspension and harvested at the time points indicated. <b>A.</b> Immunoblot for TrkB expression, α-tubulin used as loading control. <b>B.</b> NTF3 ELISA performed on medium. <i>Points,</i> mean of three biological replicates, <i>bars</i>, SEM. Cells treated with transfection reagent only (mock), scrambled negative control (neg) or miR-200c mimic (200c) and 24 hrs later plated in suspension. <b>C.</b> Cells were harvested 24 hrs later and immunoblot performed for TrkB, α-tubulin used as loading control. <b>D.</b> NTF3 ELISA performed on medium at time points indicated. <i>Points,</i> mean of three biological replicates, <i>bars</i>, SEM.