The abundance of H3K4me3 and H3K27me3 at the HCMV MIEP in shControl and shEZH2 HFFs, at the pre-immediate early stage of the infection.

<p>A. Graphical illustration of the MIEP of HCMV. Open boxes represent regions probed by ChIP for association with histone H3K27me3 and histone H3K4me3. <i>Enh</i> stands for Enhancer and <i>crs</i> stands for cis-repressive sequence. B: HFFs were transduced with either the pLKO.1 empty vector or the shEZH2 lentiviral construct. A western blot of cell lysates was probed with the EZH2 antibody. C-E. HFFs transduced with a lentivirus construct of shEZH2 or with the empty vector, were infected with HCMV (MOI 0.5 PFUs/cell). ChIPs using antibodies against H3K27me3 and H3K4me3 were analysed by qPCR using primers specific for the indicated loci (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004136#s4" target="_blank">materials and methods</a>), at 0.5 h, 1.5 h and 3 h.p.i. The results are given as mean <u>+</u> SD for triplicate Real Time PCRs. The asterisks indicate statistical significance. * indicates p<0.05 and ** indicates p<0.01.