The knockdown of GFI1 partially rescues the ability of HCMV to infect HFF cells in which EZH2 was knocked down.

<p><b>A</b>. HFFs were transduced with a TRIPZ-shEZH2 construct and with the lentiviral vector pLKO.1, or with a pLKO.1-based shGFI1construct, as indicated. Cells transduced with TRIPZ-shEZH2 and pLKO.1 (third lane), or TRIPZ-shEZH2 and pLKO.1-shGFI1 (last lane) were treated with Doxycycline, to induce shEZH2. A1. Western blotting showing the expression of EZH2 and GFI1 in HFFs transduced with the indicated constructs, in the presence or absence of Doxycycline prior to HCMV infection. A2. Western blotting, monitoring the expression of IE1 in the cells shown in F1, upon infection with HCMV. Lysates were harvested at 5 days from the start of HCMV infection. A3. Titration of the progeny virus obtained 7 days after HCMV infection of the cells shown in A1. The bars show the number of plaques per ml (mean ± SD).