UL44 and UL84 interact with the HCMV latent viral genome in CD14 (+) monocytes.

<p>(A) ChIP-Seq analysis peak map of UL44 and UL84 in HCMV latently infected CD14 (+) monocytes. UL44 or UL84 specific antibodies were used to immunoprecipitate protein-DNA complexes followed by next generation sequencing. Shown is the HCMV (Fix strain) virus genome and the location of DNA sequence reads, Red peaks = UL84 protein binding, Blue peaks = UL44 protein binding. (B) RNA4.9 interacts with components for the polycomb repressive complex. HCMV latently infected monocytes were fixed and RNA-protein complexes were immunoprecipitated with antibodies specific for EZH2, SUZ12 or UL84. RNA reverse-transcribed and cDNA was detected by PCR using primers specific for RNA4.9 or Cyclophilin A. Control immunoprecipitations were performed using an isotype control antibodies, mAb control (isotype control for SUZ12 and UL84), pAb control (isotype control for EZH2 and UL44). (C) SUZ12 and EZH2 interact with the MIEP region during latent infection. ChIP assays were performed from latently infected CD14 (+) cells using antibodies specific for SUZ12 or EZH2. Primers specific for the promoter of the MIE gene locus were used as well as control primers specific for the LUNA promoter.