<i>Amark</i> transcript localization in worker ovaries at the L₅F phase of the fifth larval instar.

<p>(A) Ovarioles showing DAPI-stained nuclei. (B) The same ovarioles as in A, but labeled with the AlexaFluor555-<i>Amark</i> sense probe (FISH negative control), shows only a reddish background coloration. (C and D) Ovarioles labeled with the AlexaFluor555-<i>Amark</i> antisense probe and DAPI: the dashed line in C highlights an ovariole with large <i>Amark</i> foci (red) in the intermediary region (arrowheads). <i>Amark</i> foci (arrowheads in D) are also concentrated at the apical region of some ovarioles (shown in higher magnification and outlined by dashed lines in D).