<i>Amark</i> transcript localization in worker ovaries at the L₅S and PP phases of the fifth larval instar.

<p>FISH with AlexaFluor555-labeled <i>Amark</i> antisense probe (red foci). Cell nuclei stained with DAPI (blue). (A) An L₅S-phase ovary showing <i>Amark</i> transcripts highly concentrated at the apical end of the ovarioles (arrows). <i>Amark</i> foci are also seen outside the apical region (arrowheads). This pattern of <i>Amark</i> labeling is generalized throughout the worker ovaries. (B and C) At the end of the fifth larval instar (PP phase) the ovary continues to show <i>Amark</i> transcripts concentrated at the apical end of the ovarioles (arrows). (D) Detail showing small-sized degenerating nuclei (arrowheads) at the tip of the ovarioles (PP phase). (E) The same ovary as seen in D, but showing <i>Amark</i> foci (arrowheads) in the region where degenerating nuclei were identified.