<i>Wolbachia</i> co-detection with DAPI and FISH in infected (A, B) versus control, uninfected ovaries (C, D).

<p><i>Wolbachia</i> appear in purple (e.g. purple arrowheads). The DAPI staining (blue or grayscale) of oocyte nuclei (e.g. *) and <i>Wolbachia</i> is lower than the fluorescence emitted by follicle cells nuclei (e.g. blue arrowheads): its observation requires overexposing the nuclei of the follicle cells (e.g. blue arrows). In infected females (A, B) <i>Wolbachia</i> FISH and DAPI signals are detected around the nuclei in infected oocytes (e.g. purple arrowheads) while in uninfected oocytes only the nuclei are labelled (white arrowheads). In uninfected females only background was observed with the FISH probe (C: e.g. yellow arrowheads) which did not co-localize with DAPI staining (D: e.g. yellow arrow). Red: <i>Wolbachia</i> FISH probe W1,2-Cy3, blue and grayscale: DAPI.