<i>Ambuffy</i> transcripts localization in the ovaries of workers at the L₅F, L₅S and PP phases of the fifth larval instar.

<p>(A) An L₅F-phase ovary incubated with DAPI for cell-nuclei staining (blue). (B) The same ovary labeled with the AlexaFluor488-<i>Ambuffy</i> sense probe (negative control) shows a greenish background coloration, but not <i>Ambuffy</i> foci. (C–F) Ovaries incubated with the AlexaFluor488-<i>Ambuffy</i> antisense probe (green foci) and DAPI. Arrowheads and arrows point to peritoneal sheath cells and ovariole cells, respectively. (C) An L₅F-phase ovary showing <i>Ambuffy</i> foci mainly in the peritoneal sheath cells involving the ovarioles. (D) An L₅S-phase ovary showing <i>Ambuffy</i> foci in the peritoneal sheath cells of a few ovarioles (seen at the right of the figure). The remaining ovarioles showed weak, or did not show, <i>Ambuffy</i> foci. (E) Detail of the ovary seen in D, highlighting the high concentration of <i>Ambuffy</i> foci mainly in the peritoneal sheath cells, but also in the ovariole cells, as shown in higher magnification. (F) A PP-phase ovary showing high concentration of <i>Ambuffy</i> foci in its basal region. The asterisk marks the basal stalk of an ovariole (evidenced by dashed lines).