Pol II accumulates near the 5′ end of genes.

<p>(A, C, E, G) The locations of primers used in this assay are shown under the gene structures. (B, D, F, H) HeLa cells were treated with 30 ng/ml of SSA for 2 hours, and the distributions of Pol II were analyzed by chromatin immunoprecipitation (ChIP). The amount of immunoprecipitated DNA fragments was measured by quantitative PCR (upper panels). The ratios between the percent input of SSA-treated cells (SSA) and methanol-treated cells (MeOH) were calculated (MeOH = 1) (lower panels). Error bars indicate s.d. (n = 4). Statistical significance was investigated by the t-test (*: p<0.05; **: p<0.01).