Increasing Pol II occupancy on <i>gTlr9</i> gene after ODN stimulation.

<p>(<b>A</b>) Oligonucleotide sets used in ChIP assay for various regions of the <i>gTlr9</i> gene are marked in Roman numerals. TSS, transcription start site; UTR, 3’ untranslated region. Occupancy of total Pol II (<b>B</b>), Pol II-CTD-Ser2 phosphorylated (Ser2-P) (<b>C</b>), and Pol II-CTD-Ser5 phosphorylated (Ser5-P) (<b>D</b>) on the <i>gTlr9</i> gene. Results are presented as the mean of triplicate samples. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163415#sec002" target="_blank">Materials and methods</a> for calculation of the percentage (vertical axis) of the ChIP data.