Ssu72 depletion causes hyperphosphorylation of the CTD on Pol II–transcribed genes.

<p>(A–C) ChIP analysis of the downstream region of the <i>GAPDH</i> gene (A), U2 and U4 snRNA genes (B), and histone H3 and H4 genes (C). (Upper) Diagrams of these genes with the coding regions, polyadenylation signals (pA), 3′ box signals, and stem-loop sequences (SL) shown as open boxes. The numbers above these genes represent distance (in nucleotides) from the end of the coding region (for <i>GAPDH</i>) or the transcription start site (for snRNA and histone genes). Dotted lines indicate the ChIP amplicons. (Lower) ChIP analysis of region 1 or 2 on the indicated genes, using antibodies against Pol II (N20), Ser2P (3E10), Ser5P (3E8), or Ser7P (4E12), in DT40 P3 (−/−) cells treated with Dox for 4 days (grey bars) or untreated (black bars). The y axe represents the fold change relative to the corresponding levels on day 0. The values for Ser2P, Ser5P or Ser7P signals were normalized by the total Pol II signal. Error bars represent standard deviations of two independent experiments.