Transcriptional activity at the <i>Itga2b</i> locus in CD41- cells.

<p>(A) Cis-element cluster analysis at the <i>Itga2b</i> locus. Vertical lines indicate probabilities that regulatory factors bind to cis-elements at these positions. The overlain curve indicates the overall probability of being within a cluster of cis-elements bound by their factors. (B) RNA polymerase II (PolII) recruitment on the <i>Itga2b</i> locus in endothelial cells (MS1). Antibody against PolII was used to perform ChIP on chip. The fold enrichments are plotted on a logarithmic scale against the position across the <i>Itga2b</i> locus and aligned to the cluster predictive plot. (C) Schematic representation of the alternative <i>Itga2b</i> TSS deduced from 5¢ RACE PCR in MS1 cells. The lower panel indicates the full-length <i>Itga2b</i> exons and the exons cloned by RACE PCR (full sequences available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043300#pone.0043300.s003" target="_blank">Figure S3</a>). (D) Cis-element cluster analysis at the <i>Mpl</i> locus. (E) Relative levels of transcription assayed by Q-PCR for <i>Itga2b</i> exons 8–9–10, 11–12 and 29–30 and for <i>Mpl</i> exons 6–7 and 11–12 in MS1 and ES, and HPC7 cells. PCR results were normalized to HPRT and compared to the level measured in HPC7 cells<b>.</b> Error bars reflect standard error or the mean (SEM).