Activation kinetics of Ano1 by Ca²⁺ and voltage.

<p>A. Representative currents of wild type Ano1, activated by 180 µM Ca²⁺ at +100 mV from a holding potential of 0 mV (voltage protocol shown above). The currents were fitted to single exponentials (superimposed in green lines) and to two exponentials (superimposed in red lines). B. The relationship of the fast (τ_fast) (dash lines) and slow (τ_slow) (solid lines) components of the activation time constant of wild type Ano1 activated by 180 nM (blue triangle), 360 nM (green square), and 1 µM (red circle) Ca²⁺ at voltages between +20 mV to +100 mV from a holding potential of 0 mV (n = 4–6 cells). C. Representative currents of wild type, ₄₄₄EEEE/AAAA₄₄₇, ΔEAVK, and Δ₄₄₄EEEEEAVKD₄₅₂ activated by 1 µM Ca²⁺ at +100 mV from a holding potential of 0 mV. The currents were fitted to single exponentials (superimpose in green lines) and to two exponentials (superimposed in red lines). D.E. The time constant of the slow (D) and fast (E) components of wild type, ₄₄₄EEEE/AAAA₄₄₇, ΔEAVK, and Δ₄₄₄EEEEEAVKD₄₅₂ activated by 1 µM Ca²⁺ at +100 mV from a holding potential of 0 mV. n = 4–8 cells; *p<0.05 vs wild type.