Activation kinetics of single alanine substituted mutations.

<p>A. Representative currents of WT, E446A, D452A, E464A, E470A, and E475A Ano1, activated by 180²⁺ at +100 mV from a holding potential of 0 mV. The currents were fitted to two exponentials (superimposed in red lines). B.C. The time constant of the slow (B) and fast (C) components of wild type and each single alanine substituted mutation activated by 180 nM Ca²⁺ at +100 mV from a holding potential of 0 mV. n = 4–8 cells.