Comparison of the sensitivities of ANO1 activation by different divalent cations.

<p>(A, B & C). Original recording traces showing the activation of ANO1 by various concentrations of divalent cations at −40 mV (black) and +40 mV (red). The saturated currents before and after the experimental concentrations of divalent cations were induced by 20 µM free Ca²⁺. (D & E) Dose-dependent activations of ANO1 by various divalent cations. Solid curves were the best fit of data points to eq. 1, with values of K_1/2′s (in µM) and Hill coefficients shown. The calcium-activation curves are the same as those shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086734#pone-0086734-g002" target="_blank">Fig. 2 B</a>. N = 4 and 5 for the Sr²⁺ and the Ba²⁺ experiments, respectively. Data points with Mg²⁺ (N = 4) are connected by straight line segments. Membrane voltages are indicated on top of each panel.