<i>In vitro</i> recall responses of splenocytes from BCG-immunized mice.

<p>6 week old BALB/c mice were immunized with 10⁷ viable BCG-SSI. Splenocytes recovered 3 weeks later, were stimulated <i>in vitro</i> at an MOI of 20 with BCG-GFP or BCG-TB1860 for 72 hrs. Con-A at 5 µg/ml was included as positive control. Supernatants were collected for the measurement of IFN-γ (A), IL-2 (B), IL-17 (C) and IL-10 (D). (E) Splenocytes stimulated for 36 hrs with the indicated BCG strains were stained for intracellular IL-17. Percentage of cells secreting IL-17 among those gated sequentially on CD3⁺, CD4⁺ and CD25⁻ are displayed. Values are mean ± S.D. from four mice.