Limited impact of Treg depletion on inflammatory cytokine production after <i>M. bovis</i> BCG infection.

<p>Intracellular cytokine production by FoxP3⁻CD4⁺ T cells was analysed in the spleen of day 7/8 and 14/15 double-depleted (black bars) or untreated DEREG mice (white bars) on day 20 after i.v. infection with 2×10⁶ CFU <i>M. bovis</i> BCG. Percentages of (A) IFN-γ, (B) IL-17A and (C) IL-10 production within live FoxP3⁻CD4⁺ T cells after PMA/ionomycin restimulation are shown. Bar graphs represent mean ± SD of 3–5 mice per group. N = 3. Statistical analysis: Mann-Whitney-U-Test. <i>*p<0.05; **p<0.01; and ***p<0.001</i>.