Cytokine production of draining lymph node cells of mice vaccinated with MGM1991, HMA or BCG vaccine.

<p>IL-2, IFN-γ TNF-α and IL-17A levels in culture supernatant of pooled inguinal lymph node cells of C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991, HMA or <i>M. bovis</i> BCG by the subcutaneous route or from unvaccinated (naïve) mice and stimulated in vitro with recombinant Ag85A (5 µg/ml), with I-A^b restricted immunodominant peptides spanning aa 241-260 of Ag85A or aa 1-20 of ESAT-6, with purified protein derivative PPD or with culture filtrate from <i>M.tb</i> culture filtrate CF (10 µg/ml). Cytokine levels are expressed in pg/ml (pooled cells from 5 animals/group).