Boosting BCG with H4-IC31® induces polyfunctional memory and effector CD4 T cells.

<p>Mice were immunized as depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039909#pone-0039909-g001" target="_blank">figure 1A</a>, and parallel with the ELISA analysis, splenocytes and PBMCs (pooled from n = five mice/group) obtained two weeks after the first H4-IC31® immunization (A) and PBMCs (pooled from n = 15 mice/group) obtained six weeks after the second immunization (B) were used for intracellular cytokine analysis by flow cytometry. Cells were stimulated with TB10.4 (upper panels) and Ag85B (lower panels). Bars represent the proportions of CD4 T cell subsets producing different cytokines as indicated on the X-axis. Background levels obtained in media-stimulated samples has been subtracted. This experiment was repeated once with similar results.