Cellular responses of rLdH2B, rLdH3, rLdH2A, rLdH4, and pooled (H2-4) of <i>L.donovani</i> in vaccinated hamsters.

<p>: (d,e,f) XTT response of mononuclear cells (lymph nodes) from normal, <i>L. donovani</i> infected and treated hamsters in response to Con A, SLD and rLdH2B, rLdH3, rLdH2A, rLdH4 and pooled (H2-4) at 10 µg/ml each. Proliferation was represented as mean OD. The data represent the means of triplicate wells ± the S.D. Nitric oxide production (µM) by (a, b, c) J774A.1 cell line. The J774 A.1 cells were primed with the supernatants of stimulated lymphocytes (3 days with mitogen and 5 days with antigens) of normal, infected and cured hamsters in response to rLdH2B, rLdH3, rLdH2A, rLdH4 and pooled (H2-4), SLD and LPS respectively at 10 µg/ml each. The estimation of NO production was done using Greiss reagent in supernatants collected from macrophage cultures 24 h after incubation and the absorbance of the reaction product was measured at 540 nm. Pooled (H2-4) shown significantly higher cellular response and nitric oxide production. Significance values indicate the difference between the SLD and rLdH2B, rLdH3, rLdH2A, rLdH4, and pooled (H2-4) stimulation (*, p<0.05; **, p<0.01; and ***, p<0.001).