Cellular responses of the combinations rLdHSP70 + rLdPDI/rLdHSP70 + rLdTPI/rLdHSP70 + rLdEL-2 in hamsters lymphocytes (lymph node cell).

<p><b>(A)</b>\ LTT response of mononuclear cells from normal, <i>L. donovani</i> infected (25 day p.c.) and treated hamsters in response to Con A, SLD and combinations (rLdHSP70 + rLdPDI/rLdHSP70 + rLdTPI/rLdHSP70 + rLdEL-2) at 10 µg/ml each. Proliferative response was represented as mean O.D of stimulated cells - mean O.D of unstimulated control cells. Each bar represents the pooled data (mean ± S.D. value) of 6 hamsters and the data represent the means of triplicate wells ± S.D. of each hamster as mean O.D of stimulated cells and mean O.D of unstimulated control cells. <b>(B)</b><b>Nitric oxide production (µM)</b>: Peritoneal macrophages were stimulated with the supernatants of stimulated lymphocytes (3 days for mitogen and 5 days for test antigens) of normal, infected and cured hamsters in response to combinations with rLdHSP70, SLD and LPS respectively at 10 µg/ml each. The estimation of NO production was done using Greiss reagent in supernatants collected from macrophage cultures 24 h after incubation and the absorbance of the reaction product was measured at 540 nm. Significance values indicate the difference between the SLD and combination with rLdHSP70 stimulation (*, <i>p</i><0.05, **, <i>p</i><0.01; ***, <i>p</i><0.001).