Cellular responses of rLdPDI of <i>L. donovani</i> in hamsters.

<p>(A) LTT response of mononuclear cells (lymph nodes) from normal, <i>L. donovani</i> infected (30 day p.c.) and treated hamsters in response to Con A, SLD and rLdPDI at 10 µg/ml each. Proliferation was represented as mean OD of stimulated culture - mean OD of unstimulated control. Each bar represents the pooled data (mean ± S.D. value) of 6 hamsters and the data represent the means of triplicate wells ± the S.D of each hamster. (B) Nitric oxide production (µM) by peritoneal macrophages of hamsters (n = 5). The peritoneal macrophages were primed with the supernatants of stimulated lymphocytes (3 days with mitogen and 5 days with antigens) of normal, infected and cured hamsters in response to rLdPDI, SLD and LPS respectively at10 µg/ml each. The estimation of NO production was done using Greiss reagent in supernatants collected from macrophage cultures 24 h after incubation and the absorbance of the reaction product was measured at 540 nm. Significance values indicate the stimulation of lymphocytes difference between the infected and cured in response to rLdPDI and compared with SLD (*, p<0.05; **, p<0.01; and ***, p<0.001).