ChAd63-MVA prime boost regimen in non-human primates.

<p>Male rhesus macaque were vaccinated i.m. with either ChAd63.ME-TRAP followed 8 weeks later by MVA.ME-TRAP (open symbols), or ChAd63.hIi-ME-TRAP followed 8 weeks later by MVA.hIi-ME-TRAP (closed symbols). Blood samples were taken on the day of vaccination, weeks 2, 4, 6, 8 following ChAd63 vaccination and 1 (9), 3 (11) and 12 (20) weeks following MVA vaccination. a.) The graphs represent the summed response to TRAP peptides over the course of the experiment for animals immunised with ME-TRAP (left) or hIi-ME-TRAP (right) vectors. The data was analysed with a 2-way anova (repeated measures) demonstrating a significant effect of the human Ii chain (p = 0.0182) and a post-hoc Bonferoni positive test to compare the two groups at each timepoint. b.) Graphs represent the response at week 2 to ME, TRAP and human Ii chain peptides measured by ELISpot at (left) and the IFN-γ TRAP specific response of CD4⁺ (centre) or CD8⁺ (right) T cells as measured by ICS at this same time. Each data set (ELISpot or ICS) was analysed with a 2-way analysis of variance and where a significant effect of human Ii was observed (ELISpot p = 0.0080), a post-hoc Bonferoni positive was performed (asterisks denote the level of significance) (****p<0.0001). c.) Graphs represent the response at week 9 to ME, TRAP and human Ii chain peptides measured by ELISpot at (left) and the IFN-γ TRAP specific response of CD4⁺ (centre) or CD8⁺ (right) T cells as measured by ICS at this same time. Each data set (ELISpot or ICS) was analysed with a 2-way analysis of variance and where a significant effect of human Ii was observed (ELISpot p = 0.0094, ICS p = 0.0274), a post-hoc Bonferoni positive was performed (asterisks denote the level of significance) (***p<0.01, *p<0.05). d.) Graphs represent the antibody response to TRAP over the course of the vaccination (left panel) or as a fold increase from the pre-vaccination timepoint. Data was analysed with a 2-way analysis of variance (repeated measure) with a significant effect of the human Ii chain observed (timecourse p = 0.0443, fold change p = 0.0341) with a post-hoc Bonferoni positive test to determine the significance at each timepoint, asterisks denote the level of significance (*p<0.05).